Applications in epithelial morphogenesis involving at pulsed UV laser

Jörg Großhans, Institute for Developmental Biochemistry, Medical School, University
of Göttingen, jgrossh@gwdg.de, Tel: 0551-3914613 (office)


SPP Workshop


Applications in epithelial morphogenesis involving at
pulsed UV laser


We will introduce methods for manipulation of cells and tissues with a pulsed UV laser.
The set up consists of an inverted spinning disc microscope equipped with a 355 nm
laser. The UV laser and the imaging laser are controlled independently.


• Ablation of junctions within an epithelial tissue. We will quantify the recoil velocity.
• Wounding of epithelial tissue. We will record Ca2+ dynamics and area oscillations
of the adjacent cells.
• Ca2+ uncaging. We will induce cell contractions in epithelial tissue by laser
induced release of Ca2+. We will quantify dynamics of cross sectional area.
• Samples and applications of the participants. Please contact the organiser to
discuss potential experiments.


Number of participants: 3
Date: 13.–15. August, 2018
Deadline for application: 15. April, 2018. Please send a brief letter of interest (one
paragraph).
Costs for travel and accommodation will be covered by central funds of the SPP.

Location: Göttingen

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