Workshop "Analyzing FRET-based molecular tension sensors"

Workshop "Analyzing FRET-based molecular tension sensors"

In this workshop, we will provide critical information on how to perform, analyze and interpret FRET-based tension sensor experiments. The workshop will cover important background information on how to design a functional tension sensor and discuss limitations and common pitfalls of this technology. We will then perform live cell experiments using TCSPC-FLIM and analyze data by custom written algorithms. Participants are encouraged to bring their own samples that can be analyzed during the workshop.

Target Group: All SPP1782 members or associates interested in FRET-based tension sensors.

Date: 16.05.2017, 1 pm to 18.05.2017, 1 pm

Venue: Max Planck Institute of Biochemistry

Group of Molecular Mechanotransduction

Am Klopferspitz 18

D-82152 Martinsried

Participant Number: 6

Organizers: C. Grashoff, A.-L. Cost

Application deadline: 01.05.2017 by e-mail to cgrasho@biochem.mpg.de (including 2-3 sentences of motivation)

Draft for a course outline

Day 1

  • Arrival and Welcome
  • Introduction into
    • Principle of FRET-based molecular tension sensor
    • Tension sensor modules – properties and calibration
    • Tension sensor design – evaluation of protein functionality
    • Tension sensor applications – data interpretation
  • Preparation of Experiments

Day 2

  • Introduction into live cell FLIM-based FRET measurements
  • Practical session: live cell TCSPC-FLIM
  • Introduction into FLIM data analysis
  • Practical session: data analysis

Day 3

  • Data analysis and data interpretation
  • Open discussion
  • Departure

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